IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2010;3(4):308-314

Original Article
Growth and cell-division in extensive (XDR) and extremely drug resistant (XXDR)
tuberculosis strains: transmission and atomic force observation

Parissa Farnia, Mohammad Reza Masjedi, Muayad Aghali Merza, Payam Tabarsei, Gennadii Konstantinovich Zhavnerko, Tengku Azmi
Ibrahim,Ho Oi Kuan, Jalladein Ghanavei, Poopak Farnia, Nikolai Nikolaevich Poleschuyk, Leonid Petrovich Titov, Parviz Owlia,Mehadi
Kazampour, Mohammad Varahram, Muaryam sheikolslami, Giovanni Battista Migliori, Ali Akbar Velayati

Mycobacteriology Research  Centre, National Research Institute of Tuberculosis and   Lung  Disease (NRITLD), WHO Collaborating
Centre, Shahid  Beheshti University (Medical Campus), Darabad, Tehran ,19556, P.O: 19575/154, Iran; The Republican Research and
Practical Centre for Epidemiology & and Microbiology, Filimonova 23, Minsk, Belarus; Institute for Chemistry of New Materials, Belarus
National Academy of Sciences, F. Skoriny 36, 220141 Minsk, Belarus; Microscopic unit, Institute of Bioscience , University Putra
Malaysia ,43400 UPM, Serdang , Selangor Darul Ehsan, Malaysia; Experimental & Animal research Laboratory, NRITLD, Shahid
Beheshti University (Medical Campus), Darabad, Tehran ,19556, P.O: 19575/154, Iran; Ministry of Health and Medical Education,
Deputy of Research, Tehran, Iran; Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; WHO
Collaborating Centre for TB and Lung Diseases, Fondazione S. Maugeri, Care and Research, Institute, Tradate, Italy.

Received August 7, 2010; accepted September 20, 2010; available online September 30, 2010

Abstract: The ultra-structure of Mycobacterium tuberculosis (MTB) was examined by transmission electronic (TEM)) and atomic force
microscopy (AFM). The study was performed to describe the morphology of susceptible, multidrug-resistant (MDR), extensively drug-
resistant (XDR) and extremely drug-resistant tuberculosis isolates (XXDR-TB) during their exponential growth phase. Four types of cell
division were observed and described. While three of them (symmetrical, asymmetrical and branching type) occurred in all isolates
studied, the fourth one was seen only in XDR and XXDR-TB bacilli. In this type of cell division, a rod shaped mother cell produced a
small round shape bacillus (0.3-0.5 µm). These round cells were different from buds or polar division, being similar to terminal
endospores, of which they did not present the typical heat resistance. Based on the present observation, we may suggest that XDR-
and XXDR-TB bacilli accommodate changes helping them to overcome the hostile environment. Viewed under AFM, the other frequently
detected shapes in MTB isolates were oval, V, Y and multi-branching filaments. These shape variation confirmed pleomorphic
phenomena in MTB populations and the specific features of pan-resistant strains.(IJCEM1008004).

Keywords: Extremely drug resistant tuberculosis, atomic force microscopy, cell –division

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Address all correspondence to:
Parissa Farnia, PhD
Mycobacteriology Research  Centre
NRITLD/UNION & WHO Collaborative Centre for TB & Lung Diseases
Shahid Beheshti University (Medical Campus)
Tehran, 19556, P.O:19575/154, IRAN
Fax No: 00982120109505