IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2010;3(4):315-331

Original Article
Selective induction of cell adhesion molecules by proinflammatory mediators in
human cardiac microvascular endothelial cells in culture

Jun Yan, Adrian D. Nunn, Regi Thomas

Ernst Felder Laboratories, Discovery Biology, Bracco Research USA, Princeton, NJ 08540, USA

Received August 25, 2010; accepted October 20, 2010; available online October 23, 2010

Abstract: The aim of this study was to determine and analyze the ability of pro-inflammatory stimuli to modulate Cell Adhesion
Molecules (CAMs) expression in cultures of human microvascular cardiac endothelial cells (HMVEC,c ).  Cell ELISA,
immunocytochemistry and flow cytometry were used to measure the CAM expressions in HMVEC,c in response to interleukins, TNF-
and LPS.  The primary finding of this study was that human microvascular endothelial cells derived from human cardiac tissue in
response to proinflammatory stimuli exhibits a unique CAM expression pattern.  Passage matched HMVEC,c from different donors
showed different CAM expression profiles, indicating inherent variability in endothelial cells.  Endothelial cells from different parts of the
vasculature are exposed to different cytokines and thus different protein expression profiles.  This cell and organ-specific differences
should be considered when interpreting findings derived from studies using endothelial cells derived from different donors and organs.
A thorough understanding of these innate differences in expression pattern of the microvasculatures of cardiac tissues might allow us
the opportunity to target these tissues selectively. (IJCEM1008005).

Keywords: P-selectin, E-selectin, VCAM-1, ICAM-1, PECAM-1, Cell adhesion molecules, LPS, TNF-alpha, inflammation, Endothelium

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Address all correspondence to:
Regi Thomas, PhD
Discovery Biology
Bracco Research USA
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