Original Article Selective induction of cell adhesion molecules by proinflammatory mediators in human cardiac microvascular endothelial cells in culture
Jun Yan, Adrian D. Nunn, Regi Thomas
Ernst Felder Laboratories, Discovery Biology, Bracco Research USA, Princeton, NJ 08540, USA
Received August 25, 2010; accepted October 20, 2010; available online October 23, 2010
Abstract: The aim of this study was to determine and analyze the ability of pro-inflammatory stimuli to modulate Cell Adhesion Molecules (CAMs) expression in cultures of human microvascular cardiac endothelial cells (HMVEC,c ). Cell ELISA, immunocytochemistry and flow cytometry were used to measure the CAM expressions in HMVEC,c in response to interleukins, TNF- and LPS. The primary finding of this study was that human microvascular endothelial cells derived from human cardiac tissue in response to proinflammatory stimuli exhibits a unique CAM expression pattern. Passage matched HMVEC,c from different donors showed different CAM expression profiles, indicating inherent variability in endothelial cells. Endothelial cells from different parts of the vasculature are exposed to different cytokines and thus different protein expression profiles. This cell and organ-specific differences should be considered when interpreting findings derived from studies using endothelial cells derived from different donors and organs. A thorough understanding of these innate differences in expression pattern of the microvasculatures of cardiac tissues might allow us the opportunity to target these tissues selectively. (IJCEM1008005).