IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2011;4(2):136-147

Original Article
Effects of Panax quinquefolium on Streptozotocin-induced diabetic rats: role of C-
peptide, nitric oxide and oxidative stress

Kamal A. Amin, Ezzat M. Awad, Mohammed A. Nagy

Biochemistry Department,  Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt; Zoology Department Faculty of
Science, El-Minia University, El-Minia , Egypt; Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.

Received January 17, 2011; accepted February 15, 2011; Epub February 30, 2011; published May 15, 2011

Abstract: Background: Insulin-dependent diabetes mellitus are at high risk for vascular disorders as hypertension and nephropathy.
Ginseng is one of the most widely used herbal medicines and is reported to have a wide range of therapeutic and pharmacological
applications for antioxidant and vasorelaxation although the mechanism is not clear. This study, aimed to evaluate hypoglycemic,
antioxidant and vasodilator effects of Panax quinquefolium aqueous ginseng extract (AGE) against streptozotocin (STZ)-induced
diabetes in male rats. Furthermore explore the role of AGE in C-peptide and nitric oxide (NO) and their relation in STZ induced diabetic
rats. Methods: Thirty White male Sprague dawley rats weighing 150-200 gm, about 4 month old were equally divided into the following:
a control group (normal, nondiabetic), a diabetic group induced by intraperitoneal (I/P) injection of STZ (non-AGE-treated) and an
AGE-treated diabetic group (STZ+AGE) (for 8 days). Serum level of urea, creatinine, glucose, insulin, C-peptide and NO were analyzed.
Activities of hepatic glucose-6-phosphatase (G6Pase), hepatic glycogen phosphorylase and the renal antioxidant enzyme, catalase
were analyzed. Also renal oxidative stress marker malondialdehyde (MDA) was measured. Results: Data showed that STZ treated rats
produced a significant increased level of serum urea, creatinine, glucose, NO and renal MDA. Also, induced significantly higher
activities of hepatic G6Pase and glycogen phosphorylase compared with controls, while give significant lowered serum insulin,
C-peptide level and renal catalase activity. Whereas treatment with AGE led to a significant amelioration in the hyperglycemia (lower the
activity of G6Pase and glycogen phosphorylase), hyperinsulinemia and oxidative stress markers. Besides declining the higher level of
renal function test and NO. Conclusions: STZ induced-diabetes (DM) associated with renal function disturbances, hypoinsulinemia,
defective antioxidant stability and increased (NO) this may have implications for the progress of DM and its related problems.
Treatment with AGE improved DM and its associated metabolic problems in different degrees. Furthermore it has insulin sensitizing,
hypoglycemic, antioxidant and vasodilator effects. Communally AGE is a way to surmount the diabetic state and it has vasodilator
effects. (IJCEM1101004).

Keywords: Panax quinquefolium, diabetes, kidney function, oxidative stress, NO, C-peptide

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Address all correspondence to:
Dr. Kamal Adel Amin
Dept of Biochemistry
Faculty of Veterinary medicine
Beni Suef University
Beni Suef, Egypt.
E-mail:
kaamin10@yahoo.com