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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2012;5(1):56-63
Original Article
Influence of fatty acids in maternal diet on atherogenesis in offspring of LDL
receptor-deficient mice
Dilênia de Oliveira Cipriano Torres, Ana Célia Oliveira Dos Santos, Amanda Karolina Soares E Silva, Patrícia Muniz Mendes Freire de
Moura, Eduardo Isidoro Carneiro Beltrão, Christina Alves Peixoto
Laboratório de Análises Clínicas - HUOC/PROCAPE; Instituto de Ciências Biológicas, Universidade de Pernambuco; Laboratório de
Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ); Departamento de Bioquímica, Universidade Federal de
Pernambuco; Centro de Tecnologias Estratégicas do Nordeste, Brazil.
Received August 9, 2011; accepted December 26, 2012; Epub January 15, 2012; Published January 31, 2012
Abstract: Aims: The present study investigated the effect of a maternal diet rich in omega-6 (E6D) or omega-9 (E9D) on atherogenesis
in the offspring of mice. Main methods: LDL receptor-deficient mice were fed a diet rich in either omega-6 (E6D) or omega-9 (E9D) for
45 days prior to mating and until the birth of the offspring, evaluating the effect on the offspring aorta in comparison to a standard diet
(STD), by immunohistochemical analysis, morphometric analysis and electron microscopy. Key findings: Hypercholesterolemic female
mice fed E6D generated offspring with high levels of total cholesterol, triglycerides (TG) and CC-chemokine ligand 2/monocyte
chemoattractant protein 1 (CCL2/MCP-1) as well as a reduction in high-density lipoprotein. The ascending aorta of these animals
exhibited an increase in arterial wall thickness as well as increased expression of CCL2/MCP-1 and vascular cell adhesion molecule
1. The ultrastructural analysis revealed severe alterations in endothelial cells. The offspring from mothers fed E9D exhibited a reduction
in TG and an increase in low-density lipoprotein. The ultrastructural analysis revealed a well-preserved aortic endothelium in these
animals. Significance: The results suggest that hypercholesterolemic mothers feed a diet rich in omega-6 predispose their offspring to
endothelial dysfunction. (IJCEM1108002).
Keywords: Maternal nutrition, maternal hypercholesterolemia, omega-6, omega-9, offspring, MCP-1, VCAM-1, atherogenesis, blood
lipids
Address all correspondence to:
Dr. Christina A Peixoto
Laboratório de Ultraestrutura
Centro de Pesquisas Aggeu Magalhães
FIOCRUZ, Av. Moraes Rego s/n
Recife, Brazil CEP: 50670-420.
Fax: (55) 81 21012516
E-mail: cpeixoto@cpqam.fiocruz.br
Original Article
Influence of fatty acids in maternal diet on atherogenesis in offspring of LDL
receptor-deficient mice
Dilênia de Oliveira Cipriano Torres, Ana Célia Oliveira Dos Santos, Amanda Karolina Soares E Silva, Patrícia Muniz Mendes Freire de
Moura, Eduardo Isidoro Carneiro Beltrão, Christina Alves Peixoto
Laboratório de Análises Clínicas - HUOC/PROCAPE; Instituto de Ciências Biológicas, Universidade de Pernambuco; Laboratório de
Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ); Departamento de Bioquímica, Universidade Federal de
Pernambuco; Centro de Tecnologias Estratégicas do Nordeste, Brazil.
Received August 9, 2011; accepted December 26, 2012; Epub January 15, 2012; Published January 31, 2012
Abstract: Aims: The present study investigated the effect of a maternal diet rich in omega-6 (E6D) or omega-9 (E9D) on atherogenesis
in the offspring of mice. Main methods: LDL receptor-deficient mice were fed a diet rich in either omega-6 (E6D) or omega-9 (E9D) for
45 days prior to mating and until the birth of the offspring, evaluating the effect on the offspring aorta in comparison to a standard diet
(STD), by immunohistochemical analysis, morphometric analysis and electron microscopy. Key findings: Hypercholesterolemic female
mice fed E6D generated offspring with high levels of total cholesterol, triglycerides (TG) and CC-chemokine ligand 2/monocyte
chemoattractant protein 1 (CCL2/MCP-1) as well as a reduction in high-density lipoprotein. The ascending aorta of these animals
exhibited an increase in arterial wall thickness as well as increased expression of CCL2/MCP-1 and vascular cell adhesion molecule
1. The ultrastructural analysis revealed severe alterations in endothelial cells. The offspring from mothers fed E9D exhibited a reduction
in TG and an increase in low-density lipoprotein. The ultrastructural analysis revealed a well-preserved aortic endothelium in these
animals. Significance: The results suggest that hypercholesterolemic mothers feed a diet rich in omega-6 predispose their offspring to
endothelial dysfunction. (IJCEM1108002).
Keywords: Maternal nutrition, maternal hypercholesterolemia, omega-6, omega-9, offspring, MCP-1, VCAM-1, atherogenesis, blood
lipids
Address all correspondence to:
Dr. Christina A Peixoto
Laboratório de Ultraestrutura
Centro de Pesquisas Aggeu Magalhães
FIOCRUZ, Av. Moraes Rego s/n
Recife, Brazil CEP: 50670-420.
Fax: (55) 81 21012516
E-mail: cpeixoto@cpqam.fiocruz.br
