IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2013;6(7):516-523

Original Article
Effects of ischemic preconditioning and iloprost on myocardial ischemia-
reperfusion damage in rats

Yasin Ay, Ibrahim Kara, Cemalettin Aydin, Nuray Kahraman Ay, Melike Elif Teker, Serkan Senol, Bekir Inan, Halil Basel, Omer Uysal,
Rahmi Zeybek

Department of Cardiovascular Surgery, Bezmialem Vakif University, Istanbul, Turkey; Department of Cardiovascular Surgery, Sakarya
University School of Medicine, Sakarya, Turkey; Department of Cardiology, Bezmialem Vakif University, Istanbul, Turkey; Department of
Pathology, Istanbul Medeniyet University, Istanbul, Turkey; Department of Biostatistics and Medical Informatics, Bezmialem Vakif
University, Istanbul, Turkey

Received May 16, 2013; Accepted June 27, 2013; Epub August 1, 2013; Published August 15, 2013

Abstract: This study investigates the effects of cardiac ischemic preconditioning and iloprost on reperfusion damage in rats with
myocardial ischemia/reperfusion. 38 male Wistar Albino rats used in this study were divided into 5 groups. The control group (Group 1)
(n=6), ischemia/reperfusion (IR) group (Group 2) (n=8), cardiac ischemic preconditioning (CIP) group (Group 3) (n=8), iloprost (ILO)
group (Group 4) (n=8), and cardiac ischemic preconditioning + iloprost (CIP+ILO) group (Group 5) (n=8). Pre-ischemia, 15 minutes
post-ischemia, 45 minutes post-reperfusion, mean blood pressure (MBP), and heart rates (HR) were recorded. The rate-pressure
product (RPP) was calculated. Post-reperfusion plasma creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin (cTn)
vlaues, and infarct size/area at risk (IS/AAR) were calculated from myocardial tissue samples. Arrhythmia and ST segment elevations
were evaluated during the ischemia and reperfusion stages. Although the MBP, HR, RPP values, biochemical parameters of CK-MB
and LDH levels, IS/AAR rates, ST segment elevation values were found to be similar in CIP and CIP+ILO groups and the IR and ILO
groups (p>0.05), CIP-containing group values had a positively meaningful difference (p<0.05) compared with the IR and ILO group.
While mild-moderate findings of damage were observed in Group 3 and Group 5, severely findings of damage were releaved in Group
2 and Group 4. The arrhythmia score of the ILO group was meaningfully lower (F: 41.4, p<0.001) than the IR group. We can conclude
that the effects of myocardial reperfusion damage can be reduced by cardiac ischemic preconditioning, intravenous iloprost reduced
the incidence of ventricular arrhythmia associated with reperfusion, and its use with CIP caused no additional changes.

Keywords: Injury, ischemia-reperfusion, ischemic preconditioning, myocardial, iloprost

Address correspondence to: Dr. Yasin Ay, Department of Cardiovascular Surgery, Bezmialem Vakif University, Istanbul, Turkey, Adnan
Menderes Bulvarı (Vatan Cad.), 34093, Fatih, Istanbul, Turkey. Phone: +90. 505 2126924; Fax: +90. 212 6217580; E-mail: