IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2013;6(9):773-778

Original Article
Cloning and expression of soluble vascular endothelial growth factors receptor-1
(sFlt-1) fragments in CHO-K1

Poopak Farnia, Mojgan Bandehpour, Jalaledin Ghanavi, Bahram Kazemi

Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Experimental Medicine and Tissue
Engineering Center, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Cellular and Molecular Research
Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Received August 31, 2013; Accepted September 15, 2013; Epub September 25, 2013; Published September 30, 2013

Abstract: Soluble form of vascular endothelial growth factor (VEGF) receptor (sFlt-1) has been considered a key target in anti
angiogenesis strategies. In this study, sFlt-1 was amplified and cloned. For recombinant production of sFlt-1 protein, Chinese hamster
ovary cell line (CHO) was used. The liposome–mediated transfection with sFlt-1 gene, were detected in sFlt-1 positive cells as early as
24 hours post-transfection. The production of sFlt-1 protein was confirmed using SDS-PAGE and immune blotting results. In present
investigation, the recombinant protein of sFlt-1 had expressed with correct folding. The system is economically applicable for large
production of sFlt protein and can be used as further therapeutic approaches in targeting the growth of solid tumor tissue.
(IJCEM1308022).

Keywords: Soluble vascular endothelial growth factors receptor, angiogenesis, CHO cell lines

Address correspondence to: Dr. Mojgan Bandehpour, Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of
Medical Sciences, Tehran, Iran. Fax: 982122439956; E-mail: m.bandehpour@sbmu.ac.ir