IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2010;4(3):169-178

Original Article
Role of oxytocin in deceleration of early atherosclerotic inflammatory processes
in adult male rats

Marwa A.Ahmed, Gehan M. ELosaily

Department of Physiology and Pathology, Faculty of Medicine, Assiut University, Egypt

Received June 14, 2011; accepted August 26, 2011; Epub September 15, 2011; published September 30, 2011

Abstract: Objective: The study aimed to examine the effect of exogenous OT administration on the inflammation and atherosclerosis in
adult male rats and its possible mechanisms. Thirty adult male rats equally divided into three groups .Control group fed regular diet;
group II fed control diet supplemented with L-methionine for 10 weeks. Group III received L-methionine and oxytocin treatment for 10
weeks. RT-PCR analysis showed that OT administration increased oxytocin receptor mRNA (2 fold, P,0.05). Blood samples were
evaluated for total homocysteine, interlukin-6(IL-6),monocyte chemoatrratant protein-1 (MCP-1) and C-reactive protein (CRP) by ELIZA.
lipid profile, nitric oxide (NO) , malondialdehyde (MDA) and reduced glutathione (GSH) were determined. Specimens from aorta were
processed for immunohistochemical staining for Aorta nuclear factor _B (NF-_κB) p65 protein. Result showed that OT administration to
group III decreased the plasma levels IL-6, MCP-1and CRP levels which were elevated in group II. Moreover, there was decrease of the
oxidative stress of group III in terms of increased plasma levels of NO and GSH and decreased plasma levels of MDA in blood. In
addition, rats of group II showed histological abnormalities manifested by thickening and ulceration of the aortic wall. Marked
increased   expression of NF-_κB in aorta of in group II was detected. However, OT administration restores the histological structure of
the aorta and decreased the expression of NF-_κB in aorta of group III similar to the control group. Conclusion: OT has anti
inflammatory pathway in atherosclerosis as it decelerates atherosclerosis by decreasing the proinflammatory responses through
many mechanisms, mainly the up regulation of its receptors. (IJCEM1106001).

Key words: Oxytocin, atherosclerosis, inflammation,  methionine

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Address all correspondence to:
Dr. Marwa A.Ahmed
Physiology Faculty of Medicine
Assiut University, Assiut, Egypt.
Tel: +20882286584; Fax: +20882332278
E-mail: m_az_ahmed@yahoo.com