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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2011;4(4):276-284
Original Article
Hypertonic/hyperoncotic solution attenuate blood-brain barrier breakdown and brain
pathology in whole body hyperthermia rats
Youtan Liu, Jing Tang, Jionxian Ye, Lifang Zhan, Shaonong Huang, Tingting Wang, Miaoning Gu
The University of Hongkong Shenzhen Hospital (Shenzhen Binhai Hospital), Shenzhen 518053, China; Department of anesthesia,
Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, China; Department of
Anesthesiology, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China; Department of Anaesthesiology, The
University of Hong Kong, Hong Kong SAR, China
Received August 22, 2011; accepted October 12, 2011; Epub October 19, 2011; Published November 30, 2011
Abstract: This study was designed to investigate the effects of hypertonic/hyperoncotic solution on blood-brain barrier damage, brain
edema and morphological changes of rats during whole body hyperthermia. 90 adult male Sprague-Dawley rats were randomized into
5 groups: Control group (a room temperature for 4 hours); Whole body hyperthermia group without solution treatment; Whole body
hyperthermia group with Ringer’s solution treatment; Whole body hyperthermia group with hydroxyethyl starch and Ringer’s solution
treatment; Whole body hyperthermia group with Hypertonic/hyperoncotic solution treatment. All rats except those of control group were
housed in a heated container and maintained at 36ºC for 3 hours until the rectal temperature reached 41-42ºC. Corresponding
solutions were administered intravenously at the beginning of whole body hyperthermia within 30 minutes as designed. Following
whole body hyperthermia, rats were subsequently cooled down for 1h. Evans blue was administered intravenously when the rectal
temperature was cooled down to 37ºC. The leakage of Evans blue-albumin and water content of brain were calculated and
morphological changes were investigated. In group with hypertonic/hyperoncotic solution treatment, brain water content and the
leakage of Evans blue-albumin were the lowest among the four whole body hyperthermia groups. Compared with the other three whole
body hyperthermia groups, in which profound to moderate damages to blood-brain barrier and brain tissue and cells were found, there
were only slight morphological changes in the group with hypertonic/hyperoncotic solutionon treatment. Treatment with
hypertonic/hyperoncotic solution appeared to attenuate the injury to blood-brain barrier and reduce brain edema and cell morphological
changes in whole body hyperthermia rats. (IJCEM1108003).
Key words: Whole body hyperthermia, hypertonic/hyperoncotic solution, brain morphological changes, brain edema, blood–brain
barrier
Full Text PDF
Address all correspondence to:
Miaoning Gu and Jionxian Ye
Department of anesthesia
Nanfang Hospital, Southern Medical University
1838 Guangzhou Avenue North, Guangzhou 510515, China.
Tel: +86-20-61641881; Fax: +86-20-61641881
E-mail: gumiaoning@126.com and yjx66@126.com
Original Article
Hypertonic/hyperoncotic solution attenuate blood-brain barrier breakdown and brain
pathology in whole body hyperthermia rats
Youtan Liu, Jing Tang, Jionxian Ye, Lifang Zhan, Shaonong Huang, Tingting Wang, Miaoning Gu
The University of Hongkong Shenzhen Hospital (Shenzhen Binhai Hospital), Shenzhen 518053, China; Department of anesthesia,
Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, China; Department of
Anesthesiology, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China; Department of Anaesthesiology, The
University of Hong Kong, Hong Kong SAR, China
Received August 22, 2011; accepted October 12, 2011; Epub October 19, 2011; Published November 30, 2011
Abstract: This study was designed to investigate the effects of hypertonic/hyperoncotic solution on blood-brain barrier damage, brain
edema and morphological changes of rats during whole body hyperthermia. 90 adult male Sprague-Dawley rats were randomized into
5 groups: Control group (a room temperature for 4 hours); Whole body hyperthermia group without solution treatment; Whole body
hyperthermia group with Ringer’s solution treatment; Whole body hyperthermia group with hydroxyethyl starch and Ringer’s solution
treatment; Whole body hyperthermia group with Hypertonic/hyperoncotic solution treatment. All rats except those of control group were
housed in a heated container and maintained at 36ºC for 3 hours until the rectal temperature reached 41-42ºC. Corresponding
solutions were administered intravenously at the beginning of whole body hyperthermia within 30 minutes as designed. Following
whole body hyperthermia, rats were subsequently cooled down for 1h. Evans blue was administered intravenously when the rectal
temperature was cooled down to 37ºC. The leakage of Evans blue-albumin and water content of brain were calculated and
morphological changes were investigated. In group with hypertonic/hyperoncotic solution treatment, brain water content and the
leakage of Evans blue-albumin were the lowest among the four whole body hyperthermia groups. Compared with the other three whole
body hyperthermia groups, in which profound to moderate damages to blood-brain barrier and brain tissue and cells were found, there
were only slight morphological changes in the group with hypertonic/hyperoncotic solutionon treatment. Treatment with
hypertonic/hyperoncotic solution appeared to attenuate the injury to blood-brain barrier and reduce brain edema and cell morphological
changes in whole body hyperthermia rats. (IJCEM1108003).
Key words: Whole body hyperthermia, hypertonic/hyperoncotic solution, brain morphological changes, brain edema, blood–brain
barrier
Full Text PDF
Address all correspondence to:
Miaoning Gu and Jionxian Ye
Department of anesthesia
Nanfang Hospital, Southern Medical University
1838 Guangzhou Avenue North, Guangzhou 510515, China.
Tel: +86-20-61641881; Fax: +86-20-61641881
E-mail: gumiaoning@126.com and yjx66@126.com
