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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2011;4(4):265-275
Original Article
Membrane type-1 matrix metalloproteinase (MT1-MMP) correlates with the
expression and activation of matrix metalloproteinase-2 (MMP-2) in inflammatory
breast cancer
Diaa Al-Raawi, Helal Abu-El-Zahab, Mohamed El-Shinawi, Mona Mostafa Mohamed
Department of Zoology, Faculty of Science- Sana’a University, Yemen; Department of Zoology, Faculty of Science- Cairo University, Giza,
12613, Egypt; Department of General Surgery, Faculty of Medicine- Ain Shams University, Cairo, 11566, Egypt.
Received September 1, 2011; accepted September 18, 2011; Epub October 11, 2011; Published November 30, 2011
Abstract: Inflammatory breast cancer (IBC) represents the most aggressive form of breast cancer, characterized by rapid progression,
characterized by dermal lymphatic emboli and extensive metastatic lymph nodes. Matrix metalloproteinases (MMPs) are proteolytic
enzymes that play an important role in cancer invasion and metastasis. Although the role of MMPs in non-IBC is well studied, little is
known about its role in IBC. Thus the goal of the present study was to 1) investigate the expression and activity levels of membrane type
matrix metalloproteinase-1 (MT1-MMP) and matrix metalloproteinase-2 and-9 (MMP-2 and MMP-9) in IBC versus non-IBC tissue
samples and; 2) test correlation between expression of MT1-MMP and pro- and active forms of MMP-2 and MMP-9. We enrolled 51
breast cancer patients, 21were diagnosed as IBC and 30 as non-IBC. Level of expression of MT1-MMP in carcinoma tissue was
assessed by immunoblot and immunohistochemistry techniques. The expression and activation of MMP-2 and MMP-9 was measured
by gelatin zymography. Our results revealed that MT1-MMP, pro-MMP-2, pro-MMP-9 and active MMP-2 were more expressed in IBC
tissue versus non-IBC. Furthermore, we found that MT1-MMP expression correlates with expression of pro-MMP-2, pro-MMP-9 and
active MMP-2 in IBC tissue samples and with MMP-9 in non-IBC tissue sample. In conclusion, our study suggests a role of MT1-MMP in
inflammatory breast cancer disease progression. (IJCEM1109005).
Key words: Inflammatory breast cancer, matrix metalloproteinases, MT1-MMP, MMP-2, MMP-9
Full Text PDF
Address all correspondence to:
Dr. Mona Mostafa Mohamed
Department of Zoology
Faculty of Science
Cairo University
12613, Cairo, Egypt
Tel. +2-02-356-766-93
Fax. +2-02-357-275-56
E-mail address: monamos@link.net
Original Article
Membrane type-1 matrix metalloproteinase (MT1-MMP) correlates with the
expression and activation of matrix metalloproteinase-2 (MMP-2) in inflammatory
breast cancer
Diaa Al-Raawi, Helal Abu-El-Zahab, Mohamed El-Shinawi, Mona Mostafa Mohamed
Department of Zoology, Faculty of Science- Sana’a University, Yemen; Department of Zoology, Faculty of Science- Cairo University, Giza,
12613, Egypt; Department of General Surgery, Faculty of Medicine- Ain Shams University, Cairo, 11566, Egypt.
Received September 1, 2011; accepted September 18, 2011; Epub October 11, 2011; Published November 30, 2011
Abstract: Inflammatory breast cancer (IBC) represents the most aggressive form of breast cancer, characterized by rapid progression,
characterized by dermal lymphatic emboli and extensive metastatic lymph nodes. Matrix metalloproteinases (MMPs) are proteolytic
enzymes that play an important role in cancer invasion and metastasis. Although the role of MMPs in non-IBC is well studied, little is
known about its role in IBC. Thus the goal of the present study was to 1) investigate the expression and activity levels of membrane type
matrix metalloproteinase-1 (MT1-MMP) and matrix metalloproteinase-2 and-9 (MMP-2 and MMP-9) in IBC versus non-IBC tissue
samples and; 2) test correlation between expression of MT1-MMP and pro- and active forms of MMP-2 and MMP-9. We enrolled 51
breast cancer patients, 21were diagnosed as IBC and 30 as non-IBC. Level of expression of MT1-MMP in carcinoma tissue was
assessed by immunoblot and immunohistochemistry techniques. The expression and activation of MMP-2 and MMP-9 was measured
by gelatin zymography. Our results revealed that MT1-MMP, pro-MMP-2, pro-MMP-9 and active MMP-2 were more expressed in IBC
tissue versus non-IBC. Furthermore, we found that MT1-MMP expression correlates with expression of pro-MMP-2, pro-MMP-9 and
active MMP-2 in IBC tissue samples and with MMP-9 in non-IBC tissue sample. In conclusion, our study suggests a role of MT1-MMP in
inflammatory breast cancer disease progression. (IJCEM1109005).
Key words: Inflammatory breast cancer, matrix metalloproteinases, MT1-MMP, MMP-2, MMP-9
Full Text PDF
Address all correspondence to:
Dr. Mona Mostafa Mohamed
Department of Zoology
Faculty of Science
Cairo University
12613, Cairo, Egypt
Tel. +2-02-356-766-93
Fax. +2-02-357-275-56
E-mail address: monamos@link.net
