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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2012;5(2):136-144
Original Article
Burn enhances toll-like receptor induced responses by circulating leukocytes
Martin G Schwacha, Qiong Zhang, Meenakshi Rani, Teresa Craig, Richard F Oppeltz
Department of Surgery, The University of Texas Health Science Center, San Antonio, TX 78229, USA.
Received January 11, 2012; accepted January 26, 2012; Epub April 6, 2012; Published April 30, 2012
Abstract: Burn and toll-like receptors (TLR) are associated with innate immune system activation, but the impact of burn on TLR-
induced inflammation responses by circulating leukocytes is unknown. To study this, C57BL/6 mice were subjected to burn (3rd
degree, 25% TBSA) or sham procedure and 1-7 days later blood was collected. Whole blood cell suspensions were incubated for 24 hr
in the presence of zymosan (TLR-2 agonist) or LPS (TLR-4 agonist). The blood cultures were responsive to TLR2 and TLR4-mediated
activation, resulting the production of IL-6, IL-10, IL-17, TNF-α, MIP-1α, MIP-1β, KC and RANTES. TLR2-induced KC and MIP-1β
production was greater in the burn group at 3-7 days post-injury, whereas IL-6, IL-10, KC and MIP-1β were greater for TLR4-induced
activation after burn. In conclusion, circulating leukocytes were responsive to TLR-induced activation and TLR-mediated inflammatory
responses were enhanced 3-7 days post-injury, as evidenced by increased production of these inflammatory mediators.
(IJCEM1201003).
Keywords: Cytokines, burn, blood, toll-like receptors, inflammation
Address all correspondence to:
Dr. Martin G. Schwacha
University of Texas Health Science Center at San Antonio
Department of Surgery Mail Code 7740, 7703 Floyd Curl Drive
San Antonio TX 78229-3900, USA.
Tel: 210-567-3623; Fax: 210-567-0003
E-mail: Schwacha@uthscsa.edu
Original Article
Burn enhances toll-like receptor induced responses by circulating leukocytes
Martin G Schwacha, Qiong Zhang, Meenakshi Rani, Teresa Craig, Richard F Oppeltz
Department of Surgery, The University of Texas Health Science Center, San Antonio, TX 78229, USA.
Received January 11, 2012; accepted January 26, 2012; Epub April 6, 2012; Published April 30, 2012
Abstract: Burn and toll-like receptors (TLR) are associated with innate immune system activation, but the impact of burn on TLR-
induced inflammation responses by circulating leukocytes is unknown. To study this, C57BL/6 mice were subjected to burn (3rd
degree, 25% TBSA) or sham procedure and 1-7 days later blood was collected. Whole blood cell suspensions were incubated for 24 hr
in the presence of zymosan (TLR-2 agonist) or LPS (TLR-4 agonist). The blood cultures were responsive to TLR2 and TLR4-mediated
activation, resulting the production of IL-6, IL-10, IL-17, TNF-α, MIP-1α, MIP-1β, KC and RANTES. TLR2-induced KC and MIP-1β
production was greater in the burn group at 3-7 days post-injury, whereas IL-6, IL-10, KC and MIP-1β were greater for TLR4-induced
activation after burn. In conclusion, circulating leukocytes were responsive to TLR-induced activation and TLR-mediated inflammatory
responses were enhanced 3-7 days post-injury, as evidenced by increased production of these inflammatory mediators.
(IJCEM1201003).
Keywords: Cytokines, burn, blood, toll-like receptors, inflammation
Address all correspondence to:
Dr. Martin G. Schwacha
University of Texas Health Science Center at San Antonio
Department of Surgery Mail Code 7740, 7703 Floyd Curl Drive
San Antonio TX 78229-3900, USA.
Tel: 210-567-3623; Fax: 210-567-0003
E-mail: Schwacha@uthscsa.edu
