 | |  | |  | |  | |  | |  | |  | |  |
| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2012;5(3):245-250
Original Article
Changes in mitochondrial DNA and its encoded products in alcoholic cirrhosis
Chun Tang, Xianchun Liang, Hongming Liu, Liping Guo, Ruxian Pi, Juntao Yang
Department of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China
Received May 17, 2012; accepted June 10, 2012; Epub June 15, 2012; Published June 30, 2012
Abstract: The purpose of this study was to investigate hepatic mitochondrial DNA (mtDNA) damage and changes in its encoded
products in patients with alcoholic cirrhosis (AC) in order to understand disease pathogenesis. We enrolled 23 patients with AC, 26
alcoholics without cirrhosis, and 25 normal subjects in this study. Hepatic mtDNA deletions were positioned using a combination of
long and accurate polymerase chain reaction (LA PCR) and gene sequencing. The mtDNA copy number was measured using
real-time quantitative PCR. The expression of the mtDNA-encoded cytochrome c oxidase 2 (cox2) was detected by western blotting. A
large deletion of bases located at positions 749-15486 was identified in hepatic mtDNA from AC patients. Moreover, the mtDNA copy
number was significantly reduced (P<0.05), and its encoded product, cox2, was significantly downregulated (P<0.05). Collectively, our
results suggest that specific deletions and reduced copy numbers of hepatic mtDNA in patients with AC is an important pathogenetic
factor. (IJCEM1205004).
Keywords: Alcoholic cirrhosis, mitochondria, mitochondrial DNA, cytochrome c oxidase 2
Address all correspondence to:
Dr. Juntao Yang
Department of Hepatobiliary Surgery
Daping Hospital, The Third Military Medical University
Chongqing 400042, China.
E-mail: juntaoyang6@gmail.com
Original Article
Changes in mitochondrial DNA and its encoded products in alcoholic cirrhosis
Chun Tang, Xianchun Liang, Hongming Liu, Liping Guo, Ruxian Pi, Juntao Yang
Department of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China
Received May 17, 2012; accepted June 10, 2012; Epub June 15, 2012; Published June 30, 2012
Abstract: The purpose of this study was to investigate hepatic mitochondrial DNA (mtDNA) damage and changes in its encoded
products in patients with alcoholic cirrhosis (AC) in order to understand disease pathogenesis. We enrolled 23 patients with AC, 26
alcoholics without cirrhosis, and 25 normal subjects in this study. Hepatic mtDNA deletions were positioned using a combination of
long and accurate polymerase chain reaction (LA PCR) and gene sequencing. The mtDNA copy number was measured using
real-time quantitative PCR. The expression of the mtDNA-encoded cytochrome c oxidase 2 (cox2) was detected by western blotting. A
large deletion of bases located at positions 749-15486 was identified in hepatic mtDNA from AC patients. Moreover, the mtDNA copy
number was significantly reduced (P<0.05), and its encoded product, cox2, was significantly downregulated (P<0.05). Collectively, our
results suggest that specific deletions and reduced copy numbers of hepatic mtDNA in patients with AC is an important pathogenetic
factor. (IJCEM1205004).
Keywords: Alcoholic cirrhosis, mitochondria, mitochondrial DNA, cytochrome c oxidase 2
Address all correspondence to:
Dr. Juntao Yang
Department of Hepatobiliary Surgery
Daping Hospital, The Third Military Medical University
Chongqing 400042, China.
E-mail: juntaoyang6@gmail.com
