IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Clin Exp Med 2013;6(2):98-104

Original Article
Toll-like receptor 4, F4/80 and pro-inflammatory cytokines in intesti-nal and
mesenteric fat tissue of Crohn’s disease

Raquel F Leal, Marciane Milanski, Maria de Lourdes S Ayrizono, Andressa Coope, Viviane S Rodrigues, Mariana Portovedo, Luiza M F
Oliveira, João J Fagundes, Cláudio S R Coy, Lício A Velloso
Coloproctology Unit, Surgery Department, University of Campinas (UNICAMP), Medical School, São Paulo, Brazil; Laboratory of Cell
Signaling, Internal Medicine Department University of Campinas (UNICAMP), Medical School, São Paulo, Brazil

Received December 15, 2012; Accepted January 11, 2013; Epub January 26, 2013; Published February 6, 2013

Abstract: Introduction: Crohn’s disease (CD) is a chronic intestinal ailment with a multifactorial etiology, whose incidence has
increased during the last three decades. Recently, a role for mesenteric fat has been proposed in CD pathophysiology, since fat
hypertrophy is detected nearby the affected intestinal area; however, there are few studies on this aspect. Aim: To evaluate inflammatory
activity in intestinal mucosa and mesenteric fat tissue of patients with CD and controls. Materials and Methods: Ten patients with
ileocecal CD and 16 patients with non-inflammatory disease (control groups) were studied. The specimens were snap-frozen and the
expression of TLR-4, F4/80, IL1-β and IL-6 were determined by immunoblot of protein extracts. TLR4 RNA level were measured using
RT-PCR. The t Test was applied (p<0.05). The local ethical committee approved the study. Results: The intestinal mucosa of CD group
had significantly higher protein levels of TLR-4, F4/80, IL-1β and IL-6 than the controls. The gene expression of TLR4 was lower in the
intestinal mucosa of CD compared to the control group. Regard the mesenteric fat tissue, there was no statistical difference related to
TLR-4, F4/80, IL-1β and IL-6 proteins expression. Conclusions: These findings may result from an up-regulation of macrophage
activation and intracellular pathways activated by bacterial antigens, which are more important in intestinal mucosa than fat tissue in
CD patients. This may represent an anomalous regulation of innate immunity and could contribute to the production of proinflammatory
mediators and disease development. (IJCEM1212015).

Keywords: Crohn’s disease, inflammatory bowel disease, innate immunity, cytokines

Address correspondence to: Dr. Rua Arquiteto José Augusto Silva, 1023, apto 42B, Mansões Santo Antônio, Zip code: 13087-570,
Campinas, Sao Paulo, Brazil. Phone: + 55-19-91375374; E-mail: raquelfl@fcm.unicamp.br; raquelleal@mpc.com.br