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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(4):259-268
Original Article
Sodium alginate/heparin composites on PVC surfaces inhibit the thrombosis and
platelet adhesion: applications in cardiac surgery
Wenqing Gao, Tingting Lin, Tong Li, Meili Yu, Xiaomin Hu, Dawei Duan
Tianjin Medical University, Tianjin, China; Department of Heart Center, The Third Central Hospital, Tianjin, China; Key Laboratory of
Artificial Cells, The Third Central Hospital, Tianjin, China. Contributed equally to the work.
Received March 8, 2013; Accepted March 28, 2013; Epub April 12, 2013; Published April 30, 2013
Abstract: Thrombosis and hemocyte damage are the main problems of applied non-coated biomaterials to cardiac surgery that
remain un-solved. The present study is aimed at the chemical modification of polyvinyl chloride (PVC) for applications in cardiac
surgery and the biological property assessment of modified PVC. Sodium alginate (SA)/heparin (HEP) composites were covalently
immobilized onto the surface of the PVC pipeline. The surface grafting density and protein adsorption were determined by ultraviolet
spectrophotometry. The surface contact angles were evaluated by contact-angle measurement, whereas the surface characteristics
were evaluated by Fourier-transform infrared spectroscopy. Blood coagulation time and platelet adhesion were measured using an
automated blood coagulation analyzer and a hemocytometer, respectively. Surface morphologies of the thrombus and platelets were
evaluated by scanning electron microscopy. The immobilization of SA/HEP reduced the contact angles of the coated surface. Protein
adsorption was reduced by the immobilization of SA. The activated partial thrombin time and thrombin time of the coated PVC were
significantly prolonged as compared with the non-coated PVC. Platelet adhesion and thrombus formation were all reduced by the
immobilization of HEP. The results revealed that the SA/HEP coating can improve the antithrombogenicity of the PVC pipeline, as well
as improve its biocompatibility and hemocompatibility, which are essential for cardiac pulmonary bypass surgery. (IJCEM1303007).
Keywords: Polyvinyl chloride (PVC), sodium alginate (SA), heparin (HEP), hemocompatibility, biocompatibility
Address correspondence to: Dr. Tong Li, Department of Heart Center, The Third Central Hospital, 83 Jintang Road, Hedong District,
Tianjin, China. Phone: 86-22-8411-2006; Fax: 22-2431-5132; E-mail: litong0806@gmail.com
Original Article
Sodium alginate/heparin composites on PVC surfaces inhibit the thrombosis and
platelet adhesion: applications in cardiac surgery
Wenqing Gao, Tingting Lin, Tong Li, Meili Yu, Xiaomin Hu, Dawei Duan
Tianjin Medical University, Tianjin, China; Department of Heart Center, The Third Central Hospital, Tianjin, China; Key Laboratory of
Artificial Cells, The Third Central Hospital, Tianjin, China. Contributed equally to the work.
Received March 8, 2013; Accepted March 28, 2013; Epub April 12, 2013; Published April 30, 2013
Abstract: Thrombosis and hemocyte damage are the main problems of applied non-coated biomaterials to cardiac surgery that
remain un-solved. The present study is aimed at the chemical modification of polyvinyl chloride (PVC) for applications in cardiac
surgery and the biological property assessment of modified PVC. Sodium alginate (SA)/heparin (HEP) composites were covalently
immobilized onto the surface of the PVC pipeline. The surface grafting density and protein adsorption were determined by ultraviolet
spectrophotometry. The surface contact angles were evaluated by contact-angle measurement, whereas the surface characteristics
were evaluated by Fourier-transform infrared spectroscopy. Blood coagulation time and platelet adhesion were measured using an
automated blood coagulation analyzer and a hemocytometer, respectively. Surface morphologies of the thrombus and platelets were
evaluated by scanning electron microscopy. The immobilization of SA/HEP reduced the contact angles of the coated surface. Protein
adsorption was reduced by the immobilization of SA. The activated partial thrombin time and thrombin time of the coated PVC were
significantly prolonged as compared with the non-coated PVC. Platelet adhesion and thrombus formation were all reduced by the
immobilization of HEP. The results revealed that the SA/HEP coating can improve the antithrombogenicity of the PVC pipeline, as well
as improve its biocompatibility and hemocompatibility, which are essential for cardiac pulmonary bypass surgery. (IJCEM1303007).
Keywords: Polyvinyl chloride (PVC), sodium alginate (SA), heparin (HEP), hemocompatibility, biocompatibility
Address correspondence to: Dr. Tong Li, Department of Heart Center, The Third Central Hospital, 83 Jintang Road, Hedong District,
Tianjin, China. Phone: 86-22-8411-2006; Fax: 22-2431-5132; E-mail: litong0806@gmail.com
