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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(6): 452-460
Original Article
Halothane increases neuronal cell death vulnerability by downregulating miR-214
and upregulating Bax
Lei Zhang, Yan Zhang
State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, 100871,
China
Received April 23, 2013; Accepted May 25, 2013; Epub June 26, 2013; Published July 1, 2013
Abstract: Inhalational general anesthesia is widely used in clinical practice, but there have been plenty of evidence shows that
application of inhalational anesthetics may increase the risk of Alzheimer’s disease (AD). Halothane is a common inhalational
anesthetics. We cultured rat primary neurons as study model to investigate cytotoxicity of halothane in neurons. We found that
halothane could enhance the cytotoxicity induced by intracellular or extracellular amyloid β (Aβ) with or without serum deprivation. In
addition, halothane induced the enhanced cytotoxicity through downregulation of miR-214 level which can lead to increasing
expression of Bax. Therefore our data suggest that halothane increases cell death induced by Aβ through increasing Bax level by
downregulating miR-214. Our study indicates a probable connection between anesthetic application and AD, while provides a new
perspective for the studies of inhalational anesthetics cytotoxicity. (IJCEM1304017).
Keywords: Halothane, miR-214, Bax, cell death, Alzheimer’s disease
Address correspondence to: Dr. Yan Zhang, College of Life Sciences, Room 219, Peking University, Beijing, 100871, China. Phone:
86-10-62754880; Fax: 86-10-62751526; E-mail: yanzhang@pku.edu.cn
Original Article
Halothane increases neuronal cell death vulnerability by downregulating miR-214
and upregulating Bax
Lei Zhang, Yan Zhang
State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, 100871,
China
Received April 23, 2013; Accepted May 25, 2013; Epub June 26, 2013; Published July 1, 2013
Abstract: Inhalational general anesthesia is widely used in clinical practice, but there have been plenty of evidence shows that
application of inhalational anesthetics may increase the risk of Alzheimer’s disease (AD). Halothane is a common inhalational
anesthetics. We cultured rat primary neurons as study model to investigate cytotoxicity of halothane in neurons. We found that
halothane could enhance the cytotoxicity induced by intracellular or extracellular amyloid β (Aβ) with or without serum deprivation. In
addition, halothane induced the enhanced cytotoxicity through downregulation of miR-214 level which can lead to increasing
expression of Bax. Therefore our data suggest that halothane increases cell death induced by Aβ through increasing Bax level by
downregulating miR-214. Our study indicates a probable connection between anesthetic application and AD, while provides a new
perspective for the studies of inhalational anesthetics cytotoxicity. (IJCEM1304017).
Keywords: Halothane, miR-214, Bax, cell death, Alzheimer’s disease
Address correspondence to: Dr. Yan Zhang, College of Life Sciences, Room 219, Peking University, Beijing, 100871, China. Phone:
86-10-62754880; Fax: 86-10-62751526; E-mail: yanzhang@pku.edu.cn
