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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(6): 431-437
Original Article
Brain-derived neurotrophic factor is up-regulated in severe acute cauda equina
syndrome dog model
Jun-Ming Tan, Jianxin Wu, Jian-Gang Shi, Guo-Dong Shi, Yan-Ling Liu, Xiao-Hong Liu, Chao-Yang Wan, De-Chun Chen, Shun-Min
Xing, Lian-Bing Shen, Lian-Shun Jia, Xiao-Jian Ye, Jia-Shun Li
Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA,
Huzhou 313000, Zhejiang Province, China; Center of Spinal Surgery of 411th Hospital of Chinese PLA, Shanghai 200081, China;
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Department of
Pathologic Laboratory of Chest Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
Received May 14, 2013; Accepted May 29, 2013; Epub June 26, 2013; Published July 1, 2013
Abstract: To determine the level of brain-derived neurotrophic factor (BDNF) in experimental dog model of severe acute cauda equina
syndrome, which was induced by multiple cauda equina constrictions throughout the entire lumbar (L), sacral (S) and coccygeal (Co)
spinal cord and their central processes of the dorsal root ganglia neurons. Adult male mongrel dogs were randomly divided into 2
groups. The experiment group (n=4) was subjected to multiple cauda equina constrictions. The control group (n=4) was subjected to
cauda equina exposure without constrictions. Level of BDNF in the spinal cord and the dorsal root ganglion cells (L7, S1-S3) was
assessed 48 hours after multiple constrictions by immunohistochemical and histopathological analyses. 48 hours after multiple
constrictions of cauda equina, up-regulation of BDNF within lumbosacral (L7-S3) spinal cord and dorsal root ganglion was observed in
experimental group as compared to control group. Our result suggests that BDNF might play a role in the inflammatory and neuropathic
pain as a result of multiple cauda equina constrictions. Regulation of BDNF level could potentially provide a therapy for treating cauda
equina syndrome. (IJCEM1305007).
Keywords: Cauda equina syndrome (CES), dorsal root ganglion (DRG), brain-derived neurotrophic factor (BDNF), multiple cauda
equina constrictions (MCEC), neurotrophic factors (NFs)
Address correspondence to: Dr. Junming Tan, Center of Trauma Repair and Reconstruction of Chinese PLA and Department of
Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China. Phone: +86 13665728018; E-mail:
Tanjunm@sina.com
Original Article
Brain-derived neurotrophic factor is up-regulated in severe acute cauda equina
syndrome dog model
Jun-Ming Tan, Jianxin Wu, Jian-Gang Shi, Guo-Dong Shi, Yan-Ling Liu, Xiao-Hong Liu, Chao-Yang Wan, De-Chun Chen, Shun-Min
Xing, Lian-Bing Shen, Lian-Shun Jia, Xiao-Jian Ye, Jia-Shun Li
Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA,
Huzhou 313000, Zhejiang Province, China; Center of Spinal Surgery of 411th Hospital of Chinese PLA, Shanghai 200081, China;
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Department of
Pathologic Laboratory of Chest Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
Received May 14, 2013; Accepted May 29, 2013; Epub June 26, 2013; Published July 1, 2013
Abstract: To determine the level of brain-derived neurotrophic factor (BDNF) in experimental dog model of severe acute cauda equina
syndrome, which was induced by multiple cauda equina constrictions throughout the entire lumbar (L), sacral (S) and coccygeal (Co)
spinal cord and their central processes of the dorsal root ganglia neurons. Adult male mongrel dogs were randomly divided into 2
groups. The experiment group (n=4) was subjected to multiple cauda equina constrictions. The control group (n=4) was subjected to
cauda equina exposure without constrictions. Level of BDNF in the spinal cord and the dorsal root ganglion cells (L7, S1-S3) was
assessed 48 hours after multiple constrictions by immunohistochemical and histopathological analyses. 48 hours after multiple
constrictions of cauda equina, up-regulation of BDNF within lumbosacral (L7-S3) spinal cord and dorsal root ganglion was observed in
experimental group as compared to control group. Our result suggests that BDNF might play a role in the inflammatory and neuropathic
pain as a result of multiple cauda equina constrictions. Regulation of BDNF level could potentially provide a therapy for treating cauda
equina syndrome. (IJCEM1305007).
Keywords: Cauda equina syndrome (CES), dorsal root ganglion (DRG), brain-derived neurotrophic factor (BDNF), multiple cauda
equina constrictions (MCEC), neurotrophic factors (NFs)
Address correspondence to: Dr. Junming Tan, Center of Trauma Repair and Reconstruction of Chinese PLA and Department of
Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China. Phone: +86 13665728018; E-mail:
Tanjunm@sina.com
