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Int J Clin Exp Med 2013;6(8):655-661
Original Article
Experimental study of comparing rhEGF with rhβFGF on improving the quality of
wound healing
Bangrong Xing, Feilong Wu, Tianzeng Li, Shaohai Qi, Julin Xie, Zhiqiang Ye
Department of Emergency, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong Province, 510630, PR China;
Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong Province, 510080, PR China
Received June 13, 2013; Accepted June 28, 2013; Epub September 1, 2013; Published September 15, 2013
Abstract: The mechanism and biological effects of comparing recombinant human epidermal growth factor (rhEGF) with recombinant
human basic fibroblast growth factor (rhβFGF) were evaluated on the model of incised wounds on ventral side of rabbit’s ears in order
to direct their medication in clinical. Total of 72 incised wounds on ventral side of 24 New Zealand rabbits’ ears were divided randomly
into two therapeutic groups (rhEGF group with 10 μg/cm2 and rhβFGF group with 100 AU/cm2) and a control group (1% silver
sulfadiazine cream, SD-Ag). The observation of wounds, the measurements of healing wound area, the calculation of wound closing
index, the histopathological examination, the electrical microscopic examination, and the expression of examining integrin β1 mRNA of
samples of three groups by in situ hybridization technique were used to evaluate the results of wound healing. The results showed that
the wound healing was accelerated in all wounds treated with rhEGF and rhβFGF, and the quality of healing wounds of two therapeutic
groups was better than that of the control group. In rhβFGF group, new granulation tissue was more than that of rhEGF group in earlier
period and metaphase of healing wound, however, the velocity of re-epithelialization in rhEGF group was faster than that of rhβFGF
group in metaphase and late period. The results indicate that rhEGF and rhβFGF can improve wound healing, but the detailed
mechanisms and the biological effects were different. rhβFGF may be used to promote the growth of granulation tissue in earlier period
and metaphase of healing wound, however, rhEGF may be used to accelerate the velocity of re-epithelialization in metaphase and late
period. That rhβFGF and rhEGF were utilized in sequence can not only accelerate the velocity of healing wound and promote the quality
of healing wound but also obtain the best ratio of effects versus value. (IJCEM1306011).
Keywords: Epidermal growth factor, basic fibroblast growth factor, wound healing
Address correspondence to: Dr. Julin Xie, Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou
Guangdong Province, 510080, PR China. Tel: 020-87755766; E-mail: xiejl90@sina.com
Original Article
Experimental study of comparing rhEGF with rhβFGF on improving the quality of
wound healing
Bangrong Xing, Feilong Wu, Tianzeng Li, Shaohai Qi, Julin Xie, Zhiqiang Ye
Department of Emergency, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong Province, 510630, PR China;
Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong Province, 510080, PR China
Received June 13, 2013; Accepted June 28, 2013; Epub September 1, 2013; Published September 15, 2013
Abstract: The mechanism and biological effects of comparing recombinant human epidermal growth factor (rhEGF) with recombinant
human basic fibroblast growth factor (rhβFGF) were evaluated on the model of incised wounds on ventral side of rabbit’s ears in order
to direct their medication in clinical. Total of 72 incised wounds on ventral side of 24 New Zealand rabbits’ ears were divided randomly
into two therapeutic groups (rhEGF group with 10 μg/cm2 and rhβFGF group with 100 AU/cm2) and a control group (1% silver
sulfadiazine cream, SD-Ag). The observation of wounds, the measurements of healing wound area, the calculation of wound closing
index, the histopathological examination, the electrical microscopic examination, and the expression of examining integrin β1 mRNA of
samples of three groups by in situ hybridization technique were used to evaluate the results of wound healing. The results showed that
the wound healing was accelerated in all wounds treated with rhEGF and rhβFGF, and the quality of healing wounds of two therapeutic
groups was better than that of the control group. In rhβFGF group, new granulation tissue was more than that of rhEGF group in earlier
period and metaphase of healing wound, however, the velocity of re-epithelialization in rhEGF group was faster than that of rhβFGF
group in metaphase and late period. The results indicate that rhEGF and rhβFGF can improve wound healing, but the detailed
mechanisms and the biological effects were different. rhβFGF may be used to promote the growth of granulation tissue in earlier period
and metaphase of healing wound, however, rhEGF may be used to accelerate the velocity of re-epithelialization in metaphase and late
period. That rhβFGF and rhEGF were utilized in sequence can not only accelerate the velocity of healing wound and promote the quality
of healing wound but also obtain the best ratio of effects versus value. (IJCEM1306011).
Keywords: Epidermal growth factor, basic fibroblast growth factor, wound healing
Address correspondence to: Dr. Julin Xie, Department of Burns, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou
Guangdong Province, 510080, PR China. Tel: 020-87755766; E-mail: xiejl90@sina.com
