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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(9):794-798
Original Article
Effect of melatonin on kidney cold ischemic preservation injury
Arif Aslaner, Omer Gunal, Hamdi Taner Turgut, Erdal Celik, Umran Yildirim, Rojbin Karakoyun Demirci, Umut Riza Gunduz, Hasan
Calis, Sami Dogan
Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey; Department of General Surgery, Marmara
University Training and Research Hospital, Istanbul, Turkey; Department of General Surgery, Derince Training and Research Hospital,
Kocaeli, Turkey; Department of Biochemistry, Isparta State Hospital, Isparta, Turkey; Department of Pathology, Duzce University, Duzce,
Turkey; Department of General Surgery, Van Training and Research Hospital, Van, Turkey
Received June 17, 2013; Accepted September 15, 2013; Epub September 25, 2013; Published September 30, 2013
Abstract: Melatonin is a potent free radical scavenger of reactive oxygen species, nitric oxide synthase inhibitor and a well-known
antioxidant secreted from pineal gland. This hormone has been reported to protect tissue from oxidative damage. In this study, we aim
to investigate the effect of melatonin on kidney cold ischemia time when added to preservation solution. Thirty male Wistar albino rats
were divided equally into three groups; Ringer Lactate (RL) solution, University of Wisconsin (UW) solution with and without melatonin.
The serum Lactate Dehydrogenase (LDH) activities of the preservation solutions at 2nd, 24th, 36th, and 48th hours were determined.
Tissue malondialdehyde (MDA) levels were also measured and a histological examination was performed at 48th hour. Melatonin that
added to preservation solution prevented enzyme elevation and decreased lipid peroxidation in preservation solution when compared
to the control group (p<0.05). The histological examination revealed that UW solution containing melatonin significantly prevented the
kidney from pathological injury (p<0.05). Melatonin added to preservation solutions such as UW solution seemed to protect the tissue
preserved effectively from cold ischemic injury for up to 48 hour. (IJCEM1306015).
Keywords: Melatonin, kidney, University of Wisconsin solution, cold preservation
Address correspondence to: Dr. Arif Aslaner, Genel Cerrahi Kliniği, Antalya Eğitim ve Araştirma Hastanesi, Varlik Mahallesi, Kazim
Karabekir Caddesi Soğuksu P.O. Box 07100, Antalya, Türkiye. Tel: +902422494400, 3387; +905314309987; Fax: +902422494462; E-
mail: arif.aslaner@antalyaeah.gov.tr; Omer Gunal, Department of General Surgery, Marmara University Training and Research
Hospital, Istanbul, Turkey. E-mail: omergunal@hotmail.com
Original Article
Effect of melatonin on kidney cold ischemic preservation injury
Arif Aslaner, Omer Gunal, Hamdi Taner Turgut, Erdal Celik, Umran Yildirim, Rojbin Karakoyun Demirci, Umut Riza Gunduz, Hasan
Calis, Sami Dogan
Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey; Department of General Surgery, Marmara
University Training and Research Hospital, Istanbul, Turkey; Department of General Surgery, Derince Training and Research Hospital,
Kocaeli, Turkey; Department of Biochemistry, Isparta State Hospital, Isparta, Turkey; Department of Pathology, Duzce University, Duzce,
Turkey; Department of General Surgery, Van Training and Research Hospital, Van, Turkey
Received June 17, 2013; Accepted September 15, 2013; Epub September 25, 2013; Published September 30, 2013
Abstract: Melatonin is a potent free radical scavenger of reactive oxygen species, nitric oxide synthase inhibitor and a well-known
antioxidant secreted from pineal gland. This hormone has been reported to protect tissue from oxidative damage. In this study, we aim
to investigate the effect of melatonin on kidney cold ischemia time when added to preservation solution. Thirty male Wistar albino rats
were divided equally into three groups; Ringer Lactate (RL) solution, University of Wisconsin (UW) solution with and without melatonin.
The serum Lactate Dehydrogenase (LDH) activities of the preservation solutions at 2nd, 24th, 36th, and 48th hours were determined.
Tissue malondialdehyde (MDA) levels were also measured and a histological examination was performed at 48th hour. Melatonin that
added to preservation solution prevented enzyme elevation and decreased lipid peroxidation in preservation solution when compared
to the control group (p<0.05). The histological examination revealed that UW solution containing melatonin significantly prevented the
kidney from pathological injury (p<0.05). Melatonin added to preservation solutions such as UW solution seemed to protect the tissue
preserved effectively from cold ischemic injury for up to 48 hour. (IJCEM1306015).
Keywords: Melatonin, kidney, University of Wisconsin solution, cold preservation
Address correspondence to: Dr. Arif Aslaner, Genel Cerrahi Kliniği, Antalya Eğitim ve Araştirma Hastanesi, Varlik Mahallesi, Kazim
Karabekir Caddesi Soğuksu P.O. Box 07100, Antalya, Türkiye. Tel: +902422494400, 3387; +905314309987; Fax: +902422494462; E-
mail: arif.aslaner@antalyaeah.gov.tr; Omer Gunal, Department of General Surgery, Marmara University Training and Research
Hospital, Istanbul, Turkey. E-mail: omergunal@hotmail.com
