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Int J Clin Exp Med 2013;6(10):951-955
Original Article
Role of mouse nerve growth factor in neural recovery following hypoxic-ischemic
brain damage
Xiaojuan Yin, Fanping Meng, Wei Wei, Aihua Li, Yu Wang, Yannan Chai, Zhichun Feng
Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No. 5, Nan Mencang, Dongcheng District, Beijing 100700,
China; Liver Cirrhosis Division, The No. 302 Hospital of PLA, Beijing 100039, China. Equal contributors.
Received September 15, 2013; Accepted October 1, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Objective: To investigate the role of mouse nerve growth factor (mNGF) in neutral repair following hypoxic-ischemic brain
damage (HIBD) in a neonatal rat model. Methods: A total of 120 neonatal rats aged 7 days were randomly divided into control group,
HIBD group and mNGF group (n=40 /group). Immediately after HIBD, mNGF was intramuscularly injected into rats in the mNGF group.
7, 14, 21 and 28 days after injection, immunohistochemistry and TUNEL staining were performed. Results: In the HIBD group, Nestin
expression was mainly found in the CA1, CA2 and CA3 regions of hippocampus. In the mNGF group, Nestin was mainly noted in the
DG and CA3 regions. 7, 14 and 28 days after treatment, significant difference was found in the Nestin expression among three groups,
but not observed 21 days after treatment. 21 and 28 days after treatment, the number of apoptotic neurons was markedly reduced when
compared with that 7 and 14 days after treatment. Conclusion: mNGF can improve the neurogenesis and inhibit neural apoptosis in the
hippocampus following HIBD in a neonatal rat model. (IJCEM1309007).
Keywords: Brain, hypoxia/ischemia, nerve growth factor, rat
Address correspondence to: Dr. Zhichun Feng, Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No. 5, Nan
Mencang, Dongcheng District, Beijing 100700, China. E-mail: drfengzc@fmmu.edu.cn
Original Article
Role of mouse nerve growth factor in neural recovery following hypoxic-ischemic
brain damage
Xiaojuan Yin, Fanping Meng, Wei Wei, Aihua Li, Yu Wang, Yannan Chai, Zhichun Feng
Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No. 5, Nan Mencang, Dongcheng District, Beijing 100700,
China; Liver Cirrhosis Division, The No. 302 Hospital of PLA, Beijing 100039, China. Equal contributors.
Received September 15, 2013; Accepted October 1, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Objective: To investigate the role of mouse nerve growth factor (mNGF) in neutral repair following hypoxic-ischemic brain
damage (HIBD) in a neonatal rat model. Methods: A total of 120 neonatal rats aged 7 days were randomly divided into control group,
HIBD group and mNGF group (n=40 /group). Immediately after HIBD, mNGF was intramuscularly injected into rats in the mNGF group.
7, 14, 21 and 28 days after injection, immunohistochemistry and TUNEL staining were performed. Results: In the HIBD group, Nestin
expression was mainly found in the CA1, CA2 and CA3 regions of hippocampus. In the mNGF group, Nestin was mainly noted in the
DG and CA3 regions. 7, 14 and 28 days after treatment, significant difference was found in the Nestin expression among three groups,
but not observed 21 days after treatment. 21 and 28 days after treatment, the number of apoptotic neurons was markedly reduced when
compared with that 7 and 14 days after treatment. Conclusion: mNGF can improve the neurogenesis and inhibit neural apoptosis in the
hippocampus following HIBD in a neonatal rat model. (IJCEM1309007).
Keywords: Brain, hypoxia/ischemia, nerve growth factor, rat
Address correspondence to: Dr. Zhichun Feng, Affiliated Bayi Children’s Hospital, Beijing Military Region General Hospital, No. 5, Nan
Mencang, Dongcheng District, Beijing 100700, China. E-mail: drfengzc@fmmu.edu.cn
