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Int J Clin Exp Med 2013;6(10):930-936
Original Article
Study of drug resistance and molecular typing of 59 cholerae01 clinical isolates
from 1984 to 2002 in Chongqing, China
Zhifeng Li, Wenzen Wang, Gang Duan, Chunyan Lian, Hua Ling, Bo Zhao, Ling Wang, Yuan Min Wang, Liangui Feng
Center for Disease Control and Prevention, No.8 Changjiang 2nd Rd, Yuzhong District, 400042, Chongqing, China; Centre for Kidney
Research, Children’s Hospital at Westmead, Sydney, NSW 2145, Australia
Received September 29, 2013; Accepted October 28, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Objective: To analyze the correlation between drug resistance and Cholerae01 clinical isolates from 1984 to 2002 in
Chongqing, China. Methods: K-B assay was applied to detect the sensitivity of 59 Cholerae01 clinical isolates (20 Ogawa, 39 Inaba) to
16 kinds of antibiotics. BioNumerics software was used for a cluster analysis of electrophoresis patterns obtained from the Not I
enzyme-cutting genomic DNA by Pulsed-field gel electrophoresis (PFGE). Results: Vibrio cholerae01 in Chongqing area, China were
highly resistant to Cotrimoxazole, Furazolidone and Streptomycin. The resistance rates were 28.81% (17/59), 61.02% (36/59) and
30.51% (18/59), respectively. While the isolates from the crowd were sensitive to Amikacin, Gentamicin, Tobramycin, Ampicillin,
Neomycin and Doxycycline, and no drug-resistant strains were observed. Conclusion: No significant changes are found in the drug
resistance of Vibrio cholerae01 from the crowd in Chongqing, China and the drug resistances of the Ogawa and the Inaba strains are
different. Vibrio cholerae01 from the crowd in Chongqing, China are highly homologous, which may be from the epidemic strains with
the same source. (IJCEM1309035).
Keywords: Vibrio cholerae01, drug-resistance, molecular typing
Address correspondence to: Dr. Liangui Feng or Dr. Zhifeng Li, Center for disease Control and Prevention, Chongqing, China. Tel:
023-68803833; E-mail: flg@cqcdc.org (Liangui Feng); qczfl@163.com (Zhifeng Li)
Original Article
Study of drug resistance and molecular typing of 59 cholerae01 clinical isolates
from 1984 to 2002 in Chongqing, China
Zhifeng Li, Wenzen Wang, Gang Duan, Chunyan Lian, Hua Ling, Bo Zhao, Ling Wang, Yuan Min Wang, Liangui Feng
Center for Disease Control and Prevention, No.8 Changjiang 2nd Rd, Yuzhong District, 400042, Chongqing, China; Centre for Kidney
Research, Children’s Hospital at Westmead, Sydney, NSW 2145, Australia
Received September 29, 2013; Accepted October 28, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Objective: To analyze the correlation between drug resistance and Cholerae01 clinical isolates from 1984 to 2002 in
Chongqing, China. Methods: K-B assay was applied to detect the sensitivity of 59 Cholerae01 clinical isolates (20 Ogawa, 39 Inaba) to
16 kinds of antibiotics. BioNumerics software was used for a cluster analysis of electrophoresis patterns obtained from the Not I
enzyme-cutting genomic DNA by Pulsed-field gel electrophoresis (PFGE). Results: Vibrio cholerae01 in Chongqing area, China were
highly resistant to Cotrimoxazole, Furazolidone and Streptomycin. The resistance rates were 28.81% (17/59), 61.02% (36/59) and
30.51% (18/59), respectively. While the isolates from the crowd were sensitive to Amikacin, Gentamicin, Tobramycin, Ampicillin,
Neomycin and Doxycycline, and no drug-resistant strains were observed. Conclusion: No significant changes are found in the drug
resistance of Vibrio cholerae01 from the crowd in Chongqing, China and the drug resistances of the Ogawa and the Inaba strains are
different. Vibrio cholerae01 from the crowd in Chongqing, China are highly homologous, which may be from the epidemic strains with
the same source. (IJCEM1309035).
Keywords: Vibrio cholerae01, drug-resistance, molecular typing
Address correspondence to: Dr. Liangui Feng or Dr. Zhifeng Li, Center for disease Control and Prevention, Chongqing, China. Tel:
023-68803833; E-mail: flg@cqcdc.org (Liangui Feng); qczfl@163.com (Zhifeng Li)
